4B6Z

Crystal structure of metallo-carboxypeptidase from Burkholderia cenocepacia

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	FAMILY M14 UNASSIGNED PEPTIDASE	polymer	405	45196.4	4	UniProt (B4EEQ5) Pfam (PF18027) Pfam (PF00246)	BURKHOLDERIA CENOCEPACIA
2	E, J, Q, X (A, B, C, D)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
3	AA, F, G, H, K... (D, A, B, C)	GLYCEROL	non-polymer		92.1	9	Chemie (GOL)
4	EA, I, P, W (D, A, B, C)	ACETATE ION	non-polymer		59.0	4	Chemie (ACT)
5	BA, CA, M, N, R... (D, B, C)	1,2-ETHANEDIOL	non-polymer		62.1	7	Chemie (EDO)
6	FA, O, V (D, B, C)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
7	DA (D)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
8	GA, HA, IA, JA (A, B, C, D)	water	water		18.0	1049	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 384 (UniProt: B4EEQ5)

PDB	External Database	Details
Met -20	-	expression tag
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Asp 20	Glu 20	engineered mutation
Ser 21	Arg 21	engineered mutation
Ser 73	His 73	engineered mutation
Gly 158	Asp 158	engineered mutation
His 210	Tyr 210	engineered mutation
Thr 296	Ala 296	engineered mutation
Glu 300	Asp 300	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	180785.6
Non-Polymers*	Number of molecules	28
	Total formula weight	1973.6
All*	Total formula weight	182759.2

*Water molecules are not included.