4B3Q

Structures of HIV-1 RT and RNA-DNA Complex Reveal a Unique RT Conformation and Substrate Interface

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	REVERSE TRANSCRIPTASE/RIBONUCLEASE H	polymer	560	64423.9	1	UniProt (P04585) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075)	HUMAN IMMUNODEFICIENCY VIRUS 1	EXORIBONUCLEASE H, P66 RT, REVERSE TRANSCRIPTASE P66 SUBUNIT
2	B (B)	P51 RT	polymer	454	52973.8	1	UniProt (P04585) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815)	HUMAN IMMUNODEFICIENCY VIRUS 1	REVERSE TRANSCRIPTASE P51 SUBUNIT
3	C (D)	PRIMER DNA	polymer	25	7670.9	1		synthetic construct
4	D (R)	TEMPLATE RNA	polymer	34	10779.5	1		synthetic construct
5	E (A)	11-CYCLOPROPYL-5,11-DIHYDRO-4-METHYL-6H-DIPYRIDO[3,2-B:2',3'-E][1,4]DIAZEPIN-6-ONE	non-polymer		266.3	1	Chemie (NVP)

Sequence modifications

A: 1 - 560 (UniProt: P04585)

PDB	External Database	Details
Gly 68	Ser 655	engineered mutation
Lys 83	Arg 670	engineered mutation
Val 411	Ile 998	engineered mutation
Ser 447	Asn 1034	engineered mutation
Lys 461	Arg 1048	engineered mutation
His 483	Tyr 1070	engineered mutation
Ile 559	Val 1146	engineered mutation

B: 1 - 440 (UniProt: P04585)

PDB	External Database	Details
Met -13	-	expression tag
Arg -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gln -1	-	expression tag
Leu 0	-	expression tag
Gly 68	Ser 655	engineered mutation
Lys 83	Arg 670	engineered mutation
Val 411	Ile 998	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	135848.1
Non-Polymers*	Number of molecules	1
	Total formula weight	266.3
All*	Total formula weight	136114.4

*Water molecules are not included.