4AW3

Structure of the mixed-function P450 MycG F286V mutant in complex with mycinamicin V in P1 space group

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	P-450-LIKE PROTEIN	polymer	417	46508.7	2	UniProt (Q59523) Pfam (PF00067)	MICROMONOSPORA GRISEORUBIDA	MYCG
2	C, K (A, B)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	2	Chemie (HEM)
3	D, L (A, B)	MYCINAMICIN V	non-polymer		711.9	2	Chemie (MYV)
4	E, F, G, H, M... (A, B)	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
5	I, J, Q, R (A, B)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
6	S, T (A, B)	water	water		18.0	518	Chemie (HOH)

Sequence modifications

A, B: 1 - 397 (UniProt: Q59523)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Val 286	Phe 286	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	93017.4
Non-Polymers*	Number of molecules	16
	Total formula weight	3793.6
All*	Total formula weight	96811.1

*Water molecules are not included.