4AV3

Crystal structure of Thermotoga Maritima sodium pumping membrane integral pyrophosphatase with metal ions in active site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	K(+)-STIMULATED PYROPHOSPHATE-ENERGIZED SODIUM PUMP	polymer	735	78217.1	2	UniProt (Q9S5X0) Pfam (PF03030) In PDB	THERMOTOGA MARITIMA	MEMBRANE INTEGRAL PYROPHOSPHATASE MEMBRANE-BOUND SODIUM-TRANSLOCATING PYROPHOSPHATASE, PYROPHOSPHATE-ENERGIZED INORGANIC PYROPHOSPHATASE, NA(+)-PPASE, TM-PPASE
2	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
3	A, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4		water	water		18.0	19	Chemie (HOH)

Sequence modifications

A, B: 2 - 726 (UniProt: Q9S5X0)

PDB	External Database	Details
Met -8	-	expression tag
Arg -7	-	expression tag
Gly -6	-	expression tag
Ser -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Leu 353	Val 353	engineered mutation
Gly 395	Ser 395	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	156434.3
Non-Polymers*	Number of molecules	4
	Total formula weight	128.8
All*	Total formula weight	156563.0

*Water molecules are not included.