4ATF
Crystal structure of inactivated mutant beta-agarase B in complex with agaro-octaose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | BETA-AGARASE B | polymer | 308 | 35744.3 | 4 | UniProt (Q9RGX8) Pfam (PF00722) | ZOBELLIA GALACTANIVORANS | BETA-AGARASE B\, FAMILY GH16 |
| 2 | E, F, G, H (E, F, G, H) | 3,6-anhydro-alpha-L-galactopyranose-(1-3)-beta-D-galactopyranose-(1-4)-3,6-anhydro-alpha-L-galactopyranose-(1-3)-beta-D-galactopyranose-(1-4)-3,6-anhydro-alpha-L-galactopyranose-(1-3)-beta-D-galactopyranose-(1-4)-3,6-anhydro-alpha-L-galactopyranose-(1-3)-beta-D-galactopyranose | branched | 1243.1 | 4 | In PDB GlyTouCan (G63389KU) | |||
| 3 | I, J, K, L (A, B, C, D) | SODIUM ION | non-polymer | 23.0 | 4 | Chemie (NA) | |||
| 4 | M, N, O, P (A, B, C, D) | water | water | 18.0 | 945 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 53 - 353 (UniProt: Q9RGX8)
| PDB | External Database | Details |
|---|---|---|
| His 47 | - | expression tag |
| His 48 | - | expression tag |
| His 49 | - | expression tag |
| His 50 | - | expression tag |
| His 51 | - | expression tag |
| His 52 | - | expression tag |
| Asp 189 | Glu 189 | engineered mutation |
| Leu 354 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 142977.1 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 4972.3 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 92.0 | |
| All* | Total formula weight | 148041.4 |
