4AMH
Influence of circular permutation on the folding pathway of a PDZ domain
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DISKS LARGE HOMOLOG 1 | polymer | 106 | 11373.0 | 2 | UniProt (Q12959) Pfam (PF00595) | HOMO SAPIENS (HUMAN) | SYNAPSE-ASSOCIATED PROTEIN 97, SAP-97, SAP97, HDLG |
| 2 | C (A) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 1 | Chemie (TRS) | |||
| 3 | D (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 4 | E, F (A, B) | water | water | 18.0 | 53 | Chemie (HOH) |
Sequence modifications
A, B: 13 - 91 (UniProt: Q12959)
A, B: 95 - 106 (UniProt: Q12959)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | expression tag |
| His 2 | - | expression tag |
| His 3 | - | expression tag |
| His 4 | - | expression tag |
| His 5 | - | expression tag |
| His 6 | - | expression tag |
| Leu 7 | - | expression tag |
| Val 8 | - | expression tag |
| Pro 9 | - | expression tag |
| Arg 10 | - | expression tag |
| Gly 11 | - | expression tag |
| Ser 12 | - | expression tag |
| Trp 28 | Ile 342 | engineered mutation |
| Ala 64 | Cys 378 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 92 | - | linker |
| Ser 93 | - | linker |
| Gly 94 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 22746.1 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 214.2 | |
| All* | Total formula weight | 22960.3 |
