4AK6

BpGH117_H302E mutant glycoside hydrolase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ANHYDRO-ALPHA-L-GALACTOSIDASE	polymer	404	45944.4	2	UniProt (B5CY74) Pfam (PF04616)	BACTEROIDES PLEBEIUS
2	C, D, E, F, G (A, B)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
3	H, I (A, B)	water	water		18.0	408	Chemie (HOH)

Sequence modifications

A, B: 22 - 402 (UniProt: B5CY74)

PDB	External Database	Details
Met -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Ser 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
Ser 9	-	expression tag
Ser 10	-	expression tag
Gly 11	-	expression tag
Leu 12	-	expression tag
Val 13	-	expression tag
Pro 14	-	expression tag
Arg 15	-	expression tag
Gly 16	-	expression tag
Ser 17	-	expression tag
His 18	-	expression tag
Met 19	-	expression tag
Ala 20	-	expression tag
Ser 21	-	expression tag
Glu 302	His 302	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	91888.8
Non-Polymers*	Number of molecules	5
	Total formula weight	121.5
All*	Total formula weight	92010.3

*Water molecules are not included.