4AAE
Crystal structure of the mutant D75N I-CreI in complex with an altered target (The four central bases, 2NN region, are composed by AGCG from 5' to 3')
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | DNA ENDONUCLEASE I-CREI | polymer | 153 | 17602.2 | 2 | UniProt (P05725) Pfam (PF00961) In PDB | CHLAMYDOMONAS REINHARDTII | I-CREI, 23S RRNA INTRON PROTEIN |
| 2 | E | 24MER DNA | polymer | 24 | 7393.8 | 1 | SYNTHETIC CONSTRUCT | ||
| 3 | G | 24MER DNA | polymer | 24 | 7344.8 | 1 | SYNTHETIC CONSTRUCT | ||
| 4 | water | water | 18.0 | 150 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 154 (UniProt: P05725)
| PDB | External Database | Details |
|---|---|---|
| Asn 75 | Asp 75 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 49943.0 | |
| All* | Total formula weight | 49943.0 |
