4A4M
Crystal structure of the light-activated constitutively active N2C, M257Y,D282C rhodopsin mutant in complex with a peptide resembling the C-terminus of the Galpha-protein subunit (GaCT)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | RHODOPSIN | polymer | 349 | 39066.6 | 1 | UniProt (P02699) Pfam (PF10413) Pfam (PF00001) | BOS TAURUS (BOVINE) | CONSTITUTIVELY ACTIVE RHODOPSIN MUTANT |
| 2 | B (B) | GUANINE NUCLEOTIDE-BINDING PROTEIN G(T) SUBUNIT ALPHA-3 | polymer | 11 | 1265.5 | 1 | UniProt (P0C7Q4) UniProt (by SIFTS) (P04695) | BOS TAURUS | GACT PEPTIDE, GUSTDUCIN ALPHA-3 CHAIN |
| 3 | C (C) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 4 | D (A) | RETINAL | non-polymer | 284.4 | 1 | Chemie (RET) | |||
| 5 | E (A) | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
| 6 | F (A) | octyl beta-D-glucopyranoside | non-polymer | 292.4 | 1 | Chemie (BOG) | |||
| 7 | G (A) | PALMITIC ACID | non-polymer | 256.4 | 1 | Chemie (PLM) | |||
| 8 | H (A) | water | water | 18.0 | 9 | Chemie (HOH) |
Sequence modifications
A: 1 - 348 (UniProt: P02699)
B: 340 - 350 (UniProt: P0C7Q4)
| PDB | External Database | Details |
|---|---|---|
| Ace 0 | - | acetylation |
| Cys 2 | Asn 2 | engineered mutation |
| Tyr 257 | Met 257 | engineered mutation |
| Cys 282 | Asp 282 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Leu 341 | Lys 345 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 40332.1 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 424.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 892.3 | |
| All* | Total formula weight | 41648.7 |
