4A4M
Crystal structure of the light-activated constitutively active N2C, M257Y,D282C rhodopsin mutant in complex with a peptide resembling the C-terminus of the Galpha-protein subunit (GaCT)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | RHODOPSIN | polymer | 349 | 39066.6 | 1 | UniProt (P02699) Pfam (PF10413) Pfam (PF00001) | BOS TAURUS (BOVINE) | CONSTITUTIVELY ACTIVE RHODOPSIN MUTANT |
| 2 | B (B) | GUANINE NUCLEOTIDE-BINDING PROTEIN G(T) SUBUNIT ALPHA-3 | polymer | 11 | 1265.5 | 1 | UniProt (P0C7Q4) UniProt (by SIFTS) (P04695) | BOS TAURUS | GACT PEPTIDE, GUSTDUCIN ALPHA-3 CHAIN |
| 3 | C (C) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 4 | D (A) | RETINAL | non-polymer | 284.4 | 1 | Chemie (RET) PubChem (1070) PubChem (23254473) PubChem (177675058) PubChem (638015) PubChem (5353924) PubChem (6436079) PubChem (6436082) PubChem (87082476) PubChem (87110449) PubChem (87110609) PubChem (88398773) PubChem (88407543) PubChem (11426127) PubChem (12227253) PubChem (12227254) PubChem (12227256) PubChem (12227257) PubChem (12227258) PubChem (12227259) PubChem (12227269) PubChem (12227270) PubChem (12227271) PubChem (5280490) PubChem (5381765) PubChem (134688327) PubChem (140510408) PubChem (145826762) PubChem (169423542) PubChem (172878320) | |||
| 5 | E (A) | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) PubChem (175) | |||
| 6 | F (A) | octyl beta-D-glucopyranoside | non-polymer | 292.4 | 1 | Chemie (BOG) | |||
| 7 | G (A) | PALMITIC ACID | non-polymer | 256.4 | 1 | Chemie (PLM) | |||
| 8 | H (A) | water | water | 18.0 | 9 | Chemie (HOH) |
Sequence modifications
A: 1 - 348 (UniProt: P02699)
B: 340 - 350 (UniProt: P0C7Q4)
| PDB | External Database | Details |
|---|---|---|
| Ace 0 | - | acetylation |
| Cys 2 | Asn 2 | engineered mutation |
| Tyr 257 | Met 257 | engineered mutation |
| Cys 282 | Asp 282 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Leu 341 | Lys 345 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 40332.1 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 424.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 892.3 | |
| All* | Total formula weight | 41648.7 |
