4A4A

CpGH89 (E483Q, E601Q), from Clostridium perfringens, in complex with its substrate GlcNAc-alpha-1,4-galactose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	ALPHA-N-ACETYLGLUCOSAMINIDASE FAMILY PROTEIN	polymer	914	104732.0	1	UniProt (Q0TST1) Pfam (PF00754) Pfam (PF12971) Pfam (PF05089) Pfam (PF12972) UniProt (by SIFTS) (Q8XM24)	CLOSTRIDIUM PERFRINGENS	FAMILY 89 GLYCOSIDE HYDROLASE
2	B (B)	2-acetamido-2-deoxy-alpha-D-glucopyranose-(1-4)-beta-D-galactopyranose	branched		383.3	1	In PDB GlyTouCan (G94473FP)
3	C, D, E, F, G... (A)	SULFATE ION	non-polymer		96.1	11	Chemie (SO4)
4	N (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
5	O, P, Q (A)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
6	R (A)	water	water		18.0	811	Chemie (HOH)

Sequence modifications

A: 26 - 916 (UniProt: Q0TST1)

PDB	External Database	Details
Met 3	-	expression tag
Gly 4	-	expression tag
Ser 5	-	expression tag
Ser 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
Ser 13	-	expression tag
Ser 14	-	expression tag
Gly 15	-	expression tag
Leu 16	-	expression tag
Val 17	-	expression tag
Pro 18	-	expression tag
Arg 19	-	expression tag
Gly 20	-	expression tag
Ser 21	-	expression tag
His 22	-	expression tag
Met 23	-	expression tag
Ala 24	-	expression tag
Ser 25	-	expression tag
Gln 483	Glu 483	engineered mutation
Gln 601	Glu 601	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	104732.0
Branched	Number of molecules	1
	Total formula weight	383.3
Non-Polymers*	Number of molecules	15
	Total formula weight	1373.1
All*	Total formula weight	106488.4

*Water molecules are not included.