4A0F

Structure of selenomethionine substituted bifunctional DAPA aminotransferase-dethiobiotin synthetase from Arabidopsis thaliana in its apo form.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ADENOSYLMETHIONINE-8-AMINO-7-OXONONANOATE AMINOTRANSFERASE	polymer	831	92193.5	2	UniProt (B0F481) Pfam (PF13500) Pfam (PF00202)	ARABIDOPSIS THALIANA (THALE CRESS)	DTB SYNTHETASE-DAPA AMINOTRANSFERASE, MITOCHONDRIAL BIFUNCTIONAL DIAMINOPELARGONATE SYNTHASE-DETHIOBIOTIN SYNTHETASE, ADENOSYLMETHIONINE-8-AMINO-7-OXONONANOATE AMINOTRANSFERASE/DETHIOBIOTIN SYNTHETASE
2	C, F (A, B)	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
3	D, E, G, H (A, B)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
4	I, J (A, B)	water	water		18.0	80	Chemie (HOH)

Sequence modifications

A, B: 1 - 811 (UniProt: B0F481)

PDB	External Database	Details
Gly -19	-	expression tag
Ser -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Val -6	-	expression tag
Pro -5	-	expression tag
Arg -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Mse 0	-	expression tag
Tyr 326	Phe 348	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	184387.0
Non-Polymers*	Number of molecules	6
	Total formula weight	878.5
All*	Total formula weight	185265.6

*Water molecules are not included.