4A08

Structure of hsDDB1-drDDB2 bound to a 13 bp CPD-duplex (purine at D-1 position) at 3.0 A resolution (CPD 1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA DAMAGE-BINDING PROTEIN 1	polymer	1159	129336.9	1	UniProt (Q16531) Pfam (PF10433) Pfam (PF03178) In PDB	HOMO SAPIENS (HUMAN)	DDB P127 SUBUNIT, DNA DAMAGE-BINDING PROTEIN A, DDBA, DAMAGE-SPECIFIC DNA-BINDING PROTEIN 1, HBV X-ASSOCIATED PROTEIN 1, XAP-1, UV-DAMAGED DNA-BINDING FACTOR, UV-DAMAGED DNA-BINDING PROTEIN 1, UV-DDB 1, XPE-BINDING FACTOR, XPE-BF, XERODERMA PIGMENTOSUM GROUP E-COMPLEMENTING PROTEIN, XPCE
2	B	DNA DAMAGE-BINDING PROTEIN 2	polymer	382	43418.1	1	UniProt (Q2YDS1) Pfam (PF00400) In PDB	DANIO RERIO (ZEBRAFISH)	DAMAGE-SPECIFIC DNA-BINDING PROTEIN 2
3	G	5'-D(*AP*CP*GP*CP*GP*AP*(TTD)P*GP*CP*GP*CP*CP*C)-3'	polymer	13	4241.8	1		synthetic construct
4	H	5'-D(*TP*GP*GP*GP*CP*GP*CP*CP*CP*TP*CP*GP*CP*G)-3'	polymer	14	4273.8	1		synthetic construct
5	A	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	2	Chemie (MES)
6	B, H	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
7		water	water		18.0	16	Chemie (HOH)

Sequence modifications

A: 1 - 1140 (UniProt: Q16531)

PDB	External Database	Details
Met -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
Ala 194	Glu 194	engineered mutation

B: 94 - 457 (UniProt: Q2YDS1)

PDB	External Database	Details
Met 76	-	expression tag
His 77	-	expression tag
His 78	-	expression tag
His 79	-	expression tag
His 80	-	expression tag
His 81	-	expression tag
His 82	-	expression tag
Arg 83	-	expression tag
Arg 84	-	expression tag
Leu 85	-	expression tag
Val 86	-	expression tag
Pro 87	-	expression tag
Arg 88	-	expression tag
Gly 89	-	expression tag
Ser 90	-	expression tag
Gly 91	-	expression tag
Gly 92	-	expression tag
Arg 93	-	expression tag
Gln 180	Leu 146	variant
Arg 214	Trp 180	variant

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	181270.5
Non-Polymers*	Number of molecules	4
	Total formula weight	470.6
All*	Total formula weight	181741.1

*Water molecules are not included.