43EI

cryo-EM structure of the extracellular domain of Anopheles stephensi IR8a-IR75k receptor in complex with octanoic acid

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ionotropic receptor coreceptor Ir8a	polymer	927	104766.5	2	UniProt (A0A182YLZ0) Pfam (PF10613) Pfam (PF00060)	Anopheles stephensi (Asian malaria mosquito)	Ionotropic receptor 8a
2	C, D (C, D)	Ionotropic glutamate receptor C-terminal domain-containing protein	polymer	657	74701.5	2	UniProt (A0A182XYX5) Pfam (PF10613) Pfam (PF00060)	Anopheles stephensi (Asian malaria mosquito)
3	E, H (E, H)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		627.6	2	In PDB GlyTouCan (G47362BJ)
4	F, G, I, J (F, G, I, J)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
5	K, L, M, N, O... (A, B, C, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	12	Chemie (NAG) PubChem (24139)
6	S, V (C, D)	OCTANOIC ACID (CAPRYLIC ACID)	non-polymer		144.2	2	Chemie (OCA) PubChem (379)

Sequence modifications

A, B: -1 - 925 (UniProt: A0A182YLZ0)

PDB	External Database	Details
Leu 62	Phe 64	conflict
Gln 81	Arg 83	conflict
Ser 106	Gly 108	conflict
Val 160	Ala 162	conflict
Asp 174	Glu 176	conflict
Val 275	Ile 277	conflict
Ala 294	Ser 296	conflict
Glu 300	Gln 302	conflict
Val 343	Ile 345	conflict
Ala 810	Thr 812	conflict

C, D: 1 - 657 (UniProt: A0A182XYX5)

PDB	External Database	Details
Ala 189	Pro 189	conflict
His 231	Gln 231	conflict
Ile 241	Val 241	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	358936.0
Branched	Number of molecules	6
	Total formula weight	2952.8
Non-Polymers*	Number of molecules	14
	Total formula weight	2942.9
All*	Total formula weight	364831.7

*Water molecules are not included.