43EF

Cryo-EM structure of Anopheles stephensi IR8a-IR75k receptor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (C, D)	Ionotropic glutamate receptor C-terminal domain-containing protein	polymer	657	74701.5	2	UniProt (A0A182XYX5) Pfam (PF10613) Pfam (PF00060)	Anopheles stephensi (Asian malaria mosquito)
2	C, D (A, B)	Ionotropic receptor coreceptor Ir8a	polymer	927	104766.5	2	UniProt (A0A182YLZ0) Pfam (PF10613) Pfam (PF00060)	Anopheles stephensi (Asian malaria mosquito)	Ionotropic receptor 8a
3	E, G (I, F)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
4	F, H (E, G)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		627.6	2	In PDB GlyTouCan (G47362BJ)
5	I, J, K, L, M... (C, D, A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	13	Chemie (NAG) PubChem (24139)

Sequence modifications

C, D: 1 - 657 (UniProt: A0A182XYX5)

PDB	External Database	Details
Ala 189	Pro 189	conflict
His 231	Gln 231	conflict
Ile 241	Val 241	conflict

A, B: 1 - 927 (UniProt: A0A182YLZ0)

PDB	External Database	Details
Leu 64	Phe 64	conflict
Gln 83	Arg 83	conflict
Ser 108	Gly 108	conflict
Val 162	Ala 162	conflict
Asp 176	Glu 176	conflict
Val 277	Ile 277	conflict
Ala 296	Ser 296	conflict
Glu 302	Gln 302	conflict
Val 345	Ile 345	conflict
Ala 812	Thr 812	conflict

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	358936.0
Branched	Number of molecules	4
	Total formula weight	2104.0
Non-Polymers*	Number of molecules	13
	Total formula weight	2875.7
All*	Total formula weight	363915.7

*Water molecules are not included.