3ZZF

Crystal structure of the amino acid kinase domain from Saccharomyces cerevisiae acetylglutamate kinase complexed with its substrate N- acetylglutamate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	ACETYLGLUTAMATE KINASE	polymer	307	34015.0	4	UniProt (Q01217) Pfam (PF00696)	SACCHAROMYCES CEREVISIAE (BAKER'S YEAST)	N-ACETYLGLUTAMATE KINASE, N-ACETYL-L-GLUTAMATE 5-PHOSPHOTRANSFERASE, NAG KINASE, AGK, PROTEIN ARG5\,6\,MITOCHONDRIAL
2	BA, CA, E, F, G... (C, A, D, B)	1,2-ETHANEDIOL	non-polymer		62.1	13	Chemie (EDO)
3	DA, I, JA, U (C, A, D, B)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
4	EA, FA, J, K, KA... (C, A, D, B)	MERCURY (II) ION	non-polymer		200.6	8	Chemie (HG)
5	GA, L, MA, X (C, A, D, B)	N-ACETYL-L-GLUTAMATE	non-polymer		189.2	4	Chemie (NLG)
6	AA, HA, M, N, NA... (B, C, A, D)	CHLORIDE ION	non-polymer		35.5	7	Chemie (CL)
7	OA, PA, QA, RA (A, B, C, D)	water	water		18.0	430	Chemie (HOH)

Sequence modifications

A, B, C, D: 58 - 356 (UniProt: Q01217)

PDB	External Database	Details
Met 50	-	expression tag
Gly 51	-	expression tag
His 52	-	expression tag
His 53	-	expression tag
His 54	-	expression tag
His 55	-	expression tag
His 56	-	expression tag
His 57	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	136060.2
Non-Polymers*	Number of molecules	36
	Total formula weight	3784.8
All*	Total formula weight	139845.0

*Water molecules are not included.