3ZWB

CRYSTAL STRUCTURE OF RAT PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE 1 (RPMFE1) COMPLEXED WITH 2TRANS-HEXENOYL-COA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	PEROXISOMAL BIFUNCTIONAL ENZYME	polymer	742	80873.3	2	UniProt (P07896) Pfam (PF00378) Pfam (PF02737) Pfam (PF00725)	RATTUS NORVEGICUS (NORWAY RAT)	PBE, PBFE, MULTIFUNCTIONAL ENZYME TYPE-1,ENOYL-COA HYDRATASE/3,2-TRANS-ENOYL-COA ISOMERASE, 3-HYDROXYACYL-COA DEHYDROGENASE
2	C, D (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3	E, I (A, B)	(2E)-Hexenoyl-CoA	non-polymer		859.6	2	Chemie (TC6)
4	F, G, H, J (A, B)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
5	K, L (A, B)	water	water		18.0	136	Chemie (HOH)

Sequence modifications

A, B: 1 - 722 (UniProt: P07896)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 123	Glu 123	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	161746.6
Non-Polymers*	Number of molecules	8
	Total formula weight	2287.7
All*	Total formula weight	164034.3

*Water molecules are not included.