3ZVI

Methylaspartate ammonia lyase from Clostridium tetanomorphum mutant L384A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	METHYLASPARTATE AMMONIA-LYASE	polymer	438	48448.2	2	UniProt (Q05514)	CLOSTRIDIUM TETANOMORPHUM	3-METHYLASPARTATE AMMONIA LYASE, BETA-METHYLASPARTASE
2	C, F, G, H, I (A, B)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
3	D, K (A, B)	PROPIONAMIDE	non-polymer		73.1	2	Chemie (ROP)
4	E, L (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	J (B)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	M, N (A, B)	water	water		18.0	796	Chemie (HOH)

Sequence modifications

A, B: 1 - 413 (UniProt: Q05514)

PDB	External Database	Details
Lys 414	-	expression tag
Leu 415	-	expression tag
Gly 416	-	expression tag
Pro 417	-	expression tag
Glu 418	-	expression tag
Gln 419	-	expression tag
Lys 420	-	expression tag
Leu 421	-	expression tag
Ile 422	-	expression tag
Ser 423	-	expression tag
Glu 424	-	expression tag
Glu 425	-	expression tag
Asp 426	-	expression tag
Leu 427	-	expression tag
Asn 428	-	expression tag
Ser 429	-	expression tag
Ala 430	-	expression tag
Val 431	-	expression tag
Asp 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag
His 438	-	expression tag
Ala 384	Leu 384	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	96896.5
Non-Polymers*	Number of molecules	10
	Total formula weight	690.7
All*	Total formula weight	97587.2

*Water molecules are not included.