3ZSN

Structure of the mixed-function P450 MycG F286A mutant in complex with mycinamicin IV

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	P-450-LIKE PROTEIN	polymer	417	46480.7	3	UniProt (Q59523) Pfam (PF00067)	MICROMONOSPORA GRISEORUBIDA	MYCG
2	D, J, P (A, B, C)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	3	Chemie (HEM)
3	E, K, Q (A, B, C)	MYCINAMICIN IV	non-polymer		695.9	3	Chemie (MIV)
4	F, L, M, R (A, B, C)	BENZAMIDINE	non-polymer		120.2	4	Chemie (BEN)
5	G, H, I, N, O... (A, B, C)	GLYCEROL	non-polymer		92.1	7	Chemie (GOL)
6	U, V, W (A, B, C)	water	water		18.0	1021	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 397 (UniProt: Q59523)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 286	Phe 286	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	139442.0
Non-Polymers*	Number of molecules	17
	Total formula weight	5062.4
All*	Total formula weight	144504.4

*Water molecules are not included.