3ZGJ

S221M V223F Y359A mutant of 4-Hydroxymandelate synthase from Streptomyces coelicolor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	4-HYDROXYPHENYLPYRUVIC ACID DIOXYGENASE	polymer	371	40236.9	2	UniProt (Q9Z4X7) Pfam (PF00903) In PDB	STREPTOMYCES COELICOLOR	4-HYDROXYMANDELATE SYNTHASE
2	A, B	COBALT (II) ION	non-polymer		58.9	2	Chemie (CO)
3	A, B	(R)-MANDELIC ACID	non-polymer		152.1	2	Chemie (RMN)
4		water	water		18.0	531	Chemie (HOH)

Sequence modifications

A, B: 1 - 371 (UniProt: Q9Z4X7)

PDB	External Database	Details
Met 221	Ser 221	engineered mutation
Phe 223	Val 223	engineered mutation
Ala 359	Tyr 359	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	80473.8
Non-Polymers*	Number of molecules	4
	Total formula weight	422.2
All*	Total formula weight	80896.0

*Water molecules are not included.