3ZGC

crystal structure of the KEAP1-NEH2 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	KELCH-LIKE ECH-ASSOCIATED PROTEIN 1	polymer	310	33840.8	2	UniProt (Q14145) Pfam (PF01344) In PDB	HOMO SAPIENS (HUMAN)	CYTOSOLIC INHIBITOR OF NRF2, INRF2, KELCH-LIKE PROTEIN 19
2	C	NUCLEAR FACTOR ERYTHROID 2-RELATED FACTOR 2	polymer	7	735.7	1	UniProt (Q16236) In PDB	HOMO SAPIENS	NF-E2-RELATED FACTOR 2, NFE2-RELATED FACTOR 2, HEBP1, NUCLEAR FACTOR, ERYTHROID DERIVED 2, LIKE 2, NEH2-DERIVED PEPTIDE
3	A, B	ACETATE ION	non-polymer		59.0	10	Chemie (ACT)
4		water	water		18.0	550	Chemie (HOH)

Sequence modifications

A, B: 321 - 609 (UniProt: Q14145)

PDB	External Database	Details
Met 300	-	expression tag
Gly 301	-	expression tag
Ser 302	-	expression tag
Ser 303	-	expression tag
His 304	-	expression tag
His 305	-	expression tag
His 306	-	expression tag
His 307	-	expression tag
His 308	-	expression tag
His 309	-	expression tag
Ser 310	-	expression tag
Ser 311	-	expression tag
Gly 312	-	expression tag
Leu 313	-	expression tag
Val 314	-	expression tag
Pro 315	-	expression tag
Arg 316	-	expression tag
Gly 317	-	expression tag
Ser 318	-	expression tag
His 319	-	expression tag
Met 320	-	expression tag
Ala 540	Glu 540	engineered mutation
Ala 542	Glu 542	engineered mutation

C: 76 - 82 (UniProt: Q16236)

PDB	External Database	Details
Gly 76	Leu 76	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	68417.3
Non-Polymers*	Number of molecules	10
	Total formula weight	590.4
All*	Total formula weight	69007.8

*Water molecules are not included.