3ZFP

Crystal structure of product-like, processed N-terminal protease Npro with internal His-Tag

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	N-TERMINAL PROTEASE NPRO	polymer	148	16662.2	1	UniProt (Q5L4B1) Pfam (PF05550) In PDB	PESTIVIRUS STRAIN D32/00_HOBI	NONSTRUCTURAL PROTEIN
2	A	MONOTHIOGLYCEROL	non-polymer		108.2	1	Chemie (SGM)
3	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4		water	water		18.0	184	Chemie (HOH)

Sequence modifications

A: 22 - 168 (UniProt: Q5L4B1)

PDB	External Database	Details
Met 21	-	expression tag
His 146	Arg 146	engineered mutation
His 147	Glu 147	engineered mutation
His 148	Gly 148	engineered mutation
His 149	Gln 149	engineered mutation
His 150	Asp 150	engineered mutation
Thr 166	Ala 166	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	16662.2
Non-Polymers*	Number of molecules	2
	Total formula weight	143.6
All*	Total formula weight	16805.8

*Water molecules are not included.