3ZFO

Crystal structure of substrate-like, unprocessed N-terminal protease Npro mutant S169P

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	N-TERMINAL PROTEASE NPRO	polymer	164	18396.1	1	UniProt (Q5L4B1) Pfam (PF05550) In PDB	PESTIVIRUS STRAIN D32/00_HOBI	NONSTRUCTURAL PROTEIN
2	A	MONOTHIOGLYCEROL	non-polymer		108.2	1	Chemie (SGM)
3	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	A	HYDROXIDE ION	non-polymer		17.0	1	Chemie (OH)
5		water	water		18.0	67	Chemie (HOH)

Sequence modifications

A: 22 - 168 (UniProt: Q5L4B1)

PDB	External Database	Details
Met 21	-	expression tag
Thr 166	Ala 166	engineered mutation
Pro 169	-	expression tag
Val 170	-	expression tag
Asp 171	-	expression tag
Lys 172	-	expression tag
Leu 173	-	expression tag
Ala 174	-	expression tag
Ala 175	-	expression tag
Ala 176	-	expression tag
Leu 177	-	expression tag
Glu 178	-	expression tag
His 179	-	expression tag
His 180	-	expression tag
His 181	-	expression tag
His 182	-	expression tag
His 183	-	expression tag
His 184	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	18396.1
Non-Polymers*	Number of molecules	3
	Total formula weight	160.6
All*	Total formula weight	18556.7

*Water molecules are not included.