3ZCG

Ascorbate peroxidase W41A-H42C mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	ASCORBATE PEROXIDASE	polymer	261	28211.8	1	UniProt (Q43758) Pfam (PF00141) In PDB	GLYCINE MAX (SOYBEAN)	CYTOSOLIC ASCORBATE PEROXIDASE 1
2	A	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	1	Chemie (HEM)
3	A	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	A	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
5	A	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
6		water	water		18.0	318	Chemie (HOH)

Sequence modifications

A: 2 - 250 (UniProt: Q43758)

PDB	External Database	Details
Met -10	-	expression tag
Arg -9	-	expression tag
Gly -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Ala 41	Trp 41	engineered mutation
Cys 42	His 42	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28211.8
Non-Polymers*	Number of molecules	5
	Total formula weight	1086.0
All*	Total formula weight	29297.8

*Water molecules are not included.