3WWG
Crystal structure of the N-glycan-deficient variant N448A of isopullulanase complexed with isopanose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D | Isopullulanase | polymer | 549 | 59995.7 | 4 | UniProt (O00105) Pfam (PF17433) Pfam (PF18841) Pfam (PF18783) Pfam (PF03718) In PDB | Aspergillus niger | |
| 2 | E | alpha-D-glucopyranose-(1-6)-beta-D-glucopyranose | branched | 342.3 | 1 | In PDB GlyTouCan (G45624LJ) | |||
| 3 | F | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 4 | C, D, A, B | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 32 | Chemie (NAG) | |||
| 5 | water | water | 18.0 | 623 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 20 - 564 (UniProt: O00105)
| PDB | External Database | Details |
|---|---|---|
| Arg 16 | - | expression tag |
| Glu 17 | - | expression tag |
| Phe 18 | - | expression tag |
| Met 19 | - | expression tag |
| Ala 448 | Asn 448 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 239982.8 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 684.6 | |
| Non-Polymers* | Number of molecules | 32 |
| Total formula weight | 7078.7 | |
| All* | Total formula weight | 247746.0 |
