3WO1
Crystal structure of Trp332Ala mutant YwfE, an L-amino acid ligase, with bound ADP-Mg-Ala
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Alanine-anticapsin ligase BacD | polymer | 470 | 51795.4 | 1 | UniProt (P39641) Pfam (PF13535) | Bacillus subtilis | Bacilysin synthetase, L-amino acid ligase |
| 2 | B (A) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 3 | C, D (A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 4 | E (A) | ALANINE | non-polymer | 89.1 | 1 | Chemie (ALA) | |||
| 5 | F (A) | water | water | 18.0 | 87 | Chemie (HOH) |
Sequence modifications
A: 4 - 468 (UniProt: P39641)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Pro 0 | - | expression tag |
| Leu 1 | - | expression tag |
| Gly 2 | - | expression tag |
| Ser 3 | - | expression tag |
| Ala 332 | Trp 332 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 51795.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 564.9 | |
| All* | Total formula weight | 52360.3 |
