3WNN
D308A mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltooctaose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Cycloisomaltooligosaccharide glucanotransferase | polymer | 710 | 79134.1 | 2 | UniProt (P94286) Pfam (PF13199) Pfam (PF16990) | Bacillus circulans | |
| 2 | C, E (C, E) | alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-beta-D-glucopyranose | branched | 1315.1 | 2 | In PDB GlyTouCan (G35793BK) | |||
| 3 | D (D) | alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose | branched | 666.6 | 1 | In PDB GlyTouCan (G80662SX) | |||
| 4 | F (F) | alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose | branched | 828.7 | 1 | In PDB GlyTouCan (G09161MH) | |||
| 5 | G, P (A, B) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 6 | H, Q (A, B) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 7 | I, J, K, L, M... (A, B) | SULFATE ION | non-polymer | 96.1 | 11 | Chemie (SO4) | |||
| 8 | O, W (A, B) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 2 | Chemie (MES) | |||
| 9 | X, Y (A, B) | water | water | 18.0 | 471 | Chemie (HOH) |
Sequence modifications
A, B: 39 - 738 (UniProt: P94286)
| PDB | External Database | Details |
|---|---|---|
| Met 37 | - | expression tag |
| Gly 38 | - | expression tag |
| Phe 278 | Ser 278 | SEE REMARK 999 |
| Ala 308 | Asp 308 | engineered mutation |
| Leu 739 | - | expression tag |
| Glu 740 | - | expression tag |
| His 741 | - | expression tag |
| His 742 | - | expression tag |
| His 743 | - | expression tag |
| His 744 | - | expression tag |
| His 745 | - | expression tag |
| His 746 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 158268.3 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 4125.6 | |
| Non-Polymers* | Number of molecules | 17 |
| Total formula weight | 1573.3 | |
| All* | Total formula weight | 163967.2 |
