3WNM
D308A mutant of Bacillus circulans T-3040 cycloisomaltooligosaccharide glucanotransferase complexed with isomaltoheptaose
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Cycloisomaltooligosaccharide glucanotransferase | polymer | 710 | 79134.1 | 1 | UniProt (P94286) Pfam (PF13199) Pfam (PF16990) In PDB | Bacillus circulans | |
2 | B | alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-beta-D-glucopyranose | branched | 1153.0 | 1 | In PDB GlyTouCan (G42588FS) | |||
3 | C | alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose-(1-6)-alpha-D-glucopyranose | branched | 666.6 | 1 | In PDB GlyTouCan (G80662SX) | |||
4 | A | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
5 | A | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
6 | A | SULFATE ION | non-polymer | 96.1 | 8 | Chemie (SO4) | |||
7 | A | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 1 | Chemie (MES) | |||
8 | water | water | 18.0 | 347 | Chemie (HOH) |
Sequence modifications
A: 39 - 738 (UniProt: P94286)
PDB | External Database | Details |
---|---|---|
Met 37 | - | expression tag |
Gly 38 | - | expression tag |
Phe 278 | Ser 278 | SEE REMARK 999 |
Ala 308 | Asp 308 | engineered mutation |
Leu 739 | - | expression tag |
Glu 740 | - | expression tag |
His 741 | - | expression tag |
His 742 | - | expression tag |
His 743 | - | expression tag |
His 744 | - | expression tag |
His 745 | - | expression tag |
His 746 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 79134.1 | |
Branched | Number of molecules | 2 |
Total formula weight | 1819.6 | |
Non-Polymers* | Number of molecules | 11 |
Total formula weight | 1026.8 | |
All* | Total formula weight | 81980.5 |