3WN5

Crystal structure of asymmetrically engineered Fc variant in complex with FcgRIIIa

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Ig gamma-1 chain C region	polymer	230	25850.2	2	UniProt (P01857) Pfam (PF07654)	Homo sapiens (human)
2	B, E (B, E)	Ig gamma-1 chain C region	polymer	230	26227.6	2	UniProt (P01857) Pfam (PF07654)	Homo sapiens (human)
3	C, F (C, F)	Low affinity immunoglobulin gamma Fc region receptor III-A	polymer	197	22626.3	2	UniProt (P08637) Pfam (PF13895)	Homo sapiens (human)	CD16a antigen, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2
4	G, H, L (G, H, L)	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1625.5	3	In PDB GlyTouCan (G33244HE)
5	I (I)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	1	In PDB GlyTouCan (G22768VO)
6	J, M (J, M)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
7	K (K)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1463.3	1	In PDB GlyTouCan (G59451NL)
8	AA, BA, CA, DA, N... (F, A, B, C, D...)	IODIDE ION	non-polymer		126.9	16	Chemie (IOD)
9	Z (F)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
10	EA, FA, GA, HA, IA... (A, B, C, D, E...)	water	water		18.0	41	Chemie (HOH)

Sequence modifications

A, D: 216 - 445 (UniProt: P01857)

PDB	External Database	Details
Ser 220	Cys 103	engineered mutation
Glu 270	Asp 153	engineered mutation
Asp 326	Lys 209	engineered mutation
Lys 330	Ala 213	engineered mutation
Glu 334	Lys 217	engineered mutation
Cys 356	Asp 239	engineered mutation
Ser 366	Thr 249	engineered mutation
Ala 368	Leu 251	engineered mutation
Val 407	Tyr 290	engineered mutation

B, E: 216 - 445 (UniProt: P01857)

PDB	External Database	Details
Ser 220	Cys 103	engineered mutation
Tyr 234	Leu 117	engineered mutation
Tyr 235	Leu 118	engineered mutation
Trp 236	Gly 119	engineered mutation
Met 239	Ser 122	engineered mutation
Asp 268	His 151	engineered mutation
Ala 298	Ser 181	engineered mutation
Asp 327	Ala 210	engineered mutation
Cys 349	Tyr 232	engineered mutation
Trp 366	Thr 249	engineered mutation

C, F: -3 - 187 (UniProt: P08637)

PDB	External Database	Details
Gln 35	Asn 56	engineered mutation
Gln 71	Asn 92	engineered mutation
Val 155	Phe 176	SEE REMARK 999
Gln 166	Asn 187	engineered mutation
His 188	-	expression tag
His 189	-	expression tag
His 190	-	expression tag
His 191	-	expression tag
His 192	-	expression tag
His 193	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	149408.2
Branched	Number of molecules	7
	Total formula weight	8099.4
Non-Polymers*	Number of molecules	17
	Total formula weight	2251.7
All*	Total formula weight	159759.3

*Water molecules are not included.