3WJZ

Orotidine 5'-monophosphate decarboxylase D75N mutant from M. thermoautotrophicus complexed with 6-amino-UMP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Orotidine 5'-phosphate decarboxylase	polymer	252	27378.4	1	UniProt (O26232) Pfam (PF00215)	Methanothermobacter thermautotrophicus	OMP decarboxylase, OMPDCase, OMPdecase
2	B (A)	6-AMINOURIDINE 5'-MONOPHOSPHATE	non-polymer		339.2	1	Chemie (NUP)
3	C (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	D, E (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	F (A)	water	water		18.0	174	Chemie (HOH)

Sequence modifications

A: 1 - 228 (UniProt: O26232)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asn 75	Asp 75	engineered mutation
Pro 101	Arg 101	SEE REMARK 999
Arg 226	Leu 226	engineered mutation
Ile 227	Asn 227	engineered mutation
Glu 229	-	expression tag
Asp 230	-	expression tag
Pro 231	-	expression tag
Ala 232	-	expression tag
Ala 233	-	expression tag
Asn 234	-	expression tag
Lys 235	-	expression tag
Ala 236	-	expression tag
Arg 237	-	expression tag
Lys 238	-	expression tag
Glu 239	-	expression tag
Ala 240	-	expression tag
Glu 241	-	expression tag
Leu 242	-	expression tag
Ala 243	-	expression tag
Ala 244	-	expression tag
Ala 245	-	expression tag
Thr 246	-	expression tag
Ala 247	-	expression tag
Glu 248	-	expression tag
Gln 249	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27378.4
Non-Polymers*	Number of molecules	4
	Total formula weight	558.8
All*	Total formula weight	27937.3

*Water molecules are not included.