3WJL

Crystal structure of IIb selective Fc variant, Fc(V12), in complex with FcgRIIb

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ig gamma-1 chain C region	polymer	230	26009.3	2	UniProt (P01857) Pfam (PF07654)	Homo sapiens (human)
2	C (C)	Low affinity immunoglobulin gamma Fc region receptor II-c	polymer	179	20257.5	1	UniProt (P31995) Pfam (PF13895)	Homo sapiens (human)	IgG Fc receptor II-c, CDw32, Fc-gamma RII-c, Fc-gamma-RIIc, FcRII-c
3	D (D)	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1479.3	1	In PDB GlyTouCan (G89993FE)
4	E (E)	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[beta-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1625.5	1	In PDB GlyTouCan (G33244HE)
5	F (C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
6	G, H, I (A, B, C)	water	water		18.0	42	Chemie (HOH)

Sequence modifications

A, B: 216 - 445 (UniProt: P01857)

PDB	External Database	Details
Ser 220	Cys 103	engineered mutation
Asp 233	Glu 116	engineered mutation
Asp 237	Gly 120	engineered mutation
Asp 238	Pro 121	engineered mutation
Asp 268	His 151	engineered mutation
Gly 271	Pro 154	engineered mutation
Arg 330	Ala 213	engineered mutation

C: 0 - 172 (UniProt: P31995)

PDB	External Database	Details
His 173	-	expression tag
His 174	-	expression tag
His 175	-	expression tag
His 176	-	expression tag
His 177	-	expression tag
His 178	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	72276.1
Branched	Number of molecules	2
	Total formula weight	3104.8
Non-Polymers*	Number of molecules	1
	Total formula weight	221.2
All*	Total formula weight	75602.1

*Water molecules are not included.