3WJC
Crystal structure of mutant nitrobindin M75L/H76L/Q96C/M148L/H158L covalently linked with [Rh(Cp-Mal)(COD)] (NB4-Rh) from Arabidopsis thaliana
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | UPF0678 fatty acid-binding protein-like protein At1g79260 | polymer | 174 | 19439.1 | 2 | UniProt (O64527) Pfam (PF08768) | Arabidopsis thaliana (mouse-ear cress thale-cress) | Nitrobindin |
| 2 | C, F (A, B) | [(1,2,5,6-eta)-cyclooctane-1,2,5,6-tetrayl]{(1,2,3,4,5-eta)-1-[2-(2,5-dioxopyrrolidin-1-yl)ethyl]cyclopentadienyl}rhodium | non-polymer | 401.3 | 2 | Chemie (RMD) | |||
| 3 | D, E, G, H (A, B) | BARIUM ION | non-polymer | 137.3 | 4 | Chemie (BA) | |||
| 4 | I, J (A, B) | water | water | 18.0 | 174 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 166 (UniProt: O64527)
| PDB | External Database | Details |
|---|---|---|
| Met -7 | - | expression tag |
| Trp -6 | - | expression tag |
| Ser -5 | - | expression tag |
| His -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Phe -1 | - | expression tag |
| Glu 0 | - | expression tag |
| Lys 1 | - | expression tag |
| Leu 75 | Met 75 | engineered mutation |
| Leu 76 | His 76 | engineered mutation |
| Cys 96 | Gln 96 | engineered mutation |
| Leu 148 | Met 148 | engineered mutation |
| Leu 158 | His 158 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 38878.1 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1351.9 | |
| All* | Total formula weight | 40230.1 |
