3WHT

Crystal structure of ERGIC-53/MCFD2, Calcium-free form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Protein ERGIC-53	polymer	246	27154.2	1	UniProt (P49257) Pfam (PF03388)	Homo sapiens (human)	ER-Golgi intermediate compartment 53 kDa protein, Gp58, Intracellular mannose-specific lectin MR60, Lectin mannose-binding 1
2	B (B)	Multiple coagulation factor deficiency protein 2	polymer	104	12056.2	1	UniProt (Q8NI22) Pfam (PF13499)	Homo sapiens (human)	Neural stem cell-derived neuronal survival protein
3	C (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	D, E (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	F, G (B)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
6	H, I (A, B)	water	water		18.0	195	Chemie (HOH)

Sequence modifications

A: 31 - 269 (UniProt: P49257)

PDB	External Database	Details
Met 24	-	expression tag
Asn 25	-	expression tag
His 26	-	expression tag
Lys 27	-	expression tag
Val 28	-	expression tag
His 29	-	expression tag
Met 30	-	expression tag

B: 67 - 146 (UniProt: Q8NI22)

PDB	External Database	Details
Met 43	-	expression tag
Gly 44	-	expression tag
His 45	-	expression tag
His 46	-	expression tag
His 47	-	expression tag
His 48	-	expression tag
His 49	-	expression tag
His 50	-	expression tag
His 51	-	expression tag
His 52	-	expression tag
His 53	-	expression tag
His 54	-	expression tag
Ser 55	-	expression tag
Ser 56	-	expression tag
Gly 57	-	expression tag
His 58	-	expression tag
Ile 59	-	expression tag
Glu 60	-	expression tag
Gly 61	-	expression tag
Arg 62	-	expression tag
His 63	-	expression tag
Met 64	-	expression tag
Leu 65	-	expression tag
Glu 66	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	39210.4
Non-Polymers*	Number of molecules	5
	Total formula weight	299.8
All*	Total formula weight	39510.2

*Water molecules are not included.