3WFZ

Crystal structure of Galacto-N-Biose/Lacto-N-Biose I Phosphorylase C236Y Mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Lacto-N-biose phosphorylase	polymer	759	85544.1	4	UniProt (E8MF13)	Bifidobacterium longum	Galacto-N-biose/lacto-N-biose I phosphorylase
2	E, F, G, H (A, B, C, D)	2-acetamido-2-deoxy-alpha-D-glucopyranose	non-polymer		221.2	4	Chemie (NDG)
3	I, J, K, L (A, B, C, D)	water	water		18.0	900	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 751 (UniProt: E8MF13)

PDB	External Database	Details
Tyr 236	Cys 236	engineered mutation
Leu 752	-	expression tag
Glu 753	-	expression tag
His 754	-	expression tag
His 755	-	expression tag
His 756	-	expression tag
His 757	-	expression tag
His 758	-	expression tag
His 759	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	342176.4
Non-Polymers*	Number of molecules	4
	Total formula weight	884.8
All*	Total formula weight	343061.2

*Water molecules are not included.