3WFA

Catalytic role of the calcium ion in GH97 inverting glycoside hydrolase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Alpha-glucosidase	polymer	726	83318.4	2	UniProt (P71094) Pfam (PF14508) Pfam (PF10566) Pfam (PF14509) UniProt (by SIFTS) (G8JZS4)	Bacteroides thetaiotaomicron	BtGH97a
2	C, E (A, B)	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
3	D, F (A, B)	{[-(BIS-CARBOXYMETHYL-AMINO)-ETHYL]-CARBOXYMETHYL-AMINO}-ACETIC ACID	non-polymer		292.2	2	Chemie (EDT)
4	G, H (A, B)	water	water		18.0	1218	Chemie (HOH)

Sequence modifications

A, B: 22 - 738 (UniProt: P71094)

PDB	External Database	Details
Met 21	-	expression tag
Leu 739	-	expression tag
Glu 740	-	expression tag
His 741	-	expression tag
His 742	-	expression tag
His 743	-	expression tag
His 744	-	expression tag
His 745	-	expression tag
His 746	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	166636.7
Non-Polymers*	Number of molecules	4
	Total formula weight	630.5
All*	Total formula weight	167267.2

*Water molecules are not included.