3WF3
Crystal structure of human beta-galactosidase mutant I51T in complex with Galactose
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Beta-galactosidase | polymer | 678 | 76606.3 | 4 | UniProt (P16278) Pfam (PF01301) Pfam (PF21317) Pfam (PF21467) In PDB | Homo sapiens (human) | Acid beta-galactosidase, Lactase, Elastin receptor 1 |
2 | C, A, D, B | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 16 | Chemie (NAG) | |||
3 | C, A, D, B | beta-D-galactopyranose | non-polymer | 180.2 | 4 | Chemie (GAL) | |||
4 | C, A, D, B | CHLORIDE ION | non-polymer | 35.5 | 4 | Chemie (CL) | |||
5 | C, A, D, B | SULFATE ION | non-polymer | 96.1 | 8 | Chemie (SO4) | |||
6 | C, A, D, B | 1,2-ETHANEDIOL | non-polymer | 62.1 | 8 | Chemie (EDO) | |||
7 | water | water | 18.0 | 1428 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 24 - 677 (UniProt: P16278)
PDB | External Database | Details |
---|---|---|
Glu 0 | - | expression tag |
Ala 1 | - | expression tag |
Glu 2 | - | expression tag |
Ala 3 | - | expression tag |
Tyr 4 | - | expression tag |
Val 5 | - | expression tag |
Glu 6 | - | expression tag |
Phe 7 | - | expression tag |
His 8 | - | expression tag |
His 9 | - | expression tag |
His 10 | - | expression tag |
His 11 | - | expression tag |
His 12 | - | expression tag |
His 13 | - | expression tag |
Asp 14 | - | expression tag |
Tyr 15 | - | expression tag |
Lys 16 | - | expression tag |
Asp 17 | - | expression tag |
Asp 18 | - | expression tag |
Asp 19 | - | expression tag |
Asp 20 | - | expression tag |
Lys 21 | - | expression tag |
Thr 22 | - | expression tag |
Ser 23 | - | expression tag |
Thr 51 | Ile 51 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 306425.2 | |
Non-Polymers* | Number of molecules | 40 |
Total formula weight | 5666.8 | |
All* | Total formula weight | 312092.0 |