3WAZ
Crystal structure of a restriction enzyme PabI in complex with DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Putative uncharacterized protein | polymer | 220 | 25340.1 | 2 | UniProt (Q9V2B6) Pfam (PF09522) | Pyrococcus abyssi | Restriction enzyme PabI |
| 2 | C, D (C, D) | DNA (5'-D(*GP*CP*AP*TP*AP*GP*CP*TP*GP*TP*(ORP)P*CP*AP*GP*CP*TP*AP*TP*GP*C)-3') | polymer | 20 | 6016.9 | 2 | synthetic construct | ||
| 3 | E, F (A, B) | ADENINE | non-polymer | 135.1 | 2 | Chemie (ADE) |
Sequence modifications
A, B: 8 - 226 (UniProt: Q9V2B6)
| PDB | External Database | Details |
|---|---|---|
| Met 7 | - | expression tag |
| Ala 154 | Lys 154 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 62714.0 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 270.3 | |
| All* | Total formula weight | 62984.3 |
