3W99

Crystal Structure of Human Nucleosome Core Particle lacking H4 N-terminal region

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Histone H3.1	polymer	139	15719.4	2	UniProt (P68431) Pfam (PF00125) In PDB	Homo sapiens (Human)	Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l
2	B, F	Histone H4	polymer	105	11869.0	2	UniProt (P62805) Pfam (PF15511) In PDB	Homo sapiens (Human)
3	C, G	Histone H2A type 1-B/E	polymer	133	14447.8	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211) In PDB	Homo sapiens (Human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	D, H	Histone H2B type 1-J	polymer	129	14217.5	2	UniProt (P06899) Pfam (PF00125) In PDB	Homo sapiens (Human)	Histone H2B.1,Histone H2B.r,H2B/r
5	I, J	146-mer DNA	polymer	146	45053.9	2		Homo sapiens
6	E	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)

Sequence modifications

A, E: 0 - 135 (UniProt: P68431)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

B, F: 16 - 102 (UniProt: P62805)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Met 1	-	expression tag
Val 2	-	expression tag
Asp 3	-	expression tag
Leu 4	-	expression tag
Gln 5	-	expression tag
Ala 6	-	expression tag
Ala 7	-	expression tag
Ala 8	-	expression tag
Asn 9	-	expression tag
Ser 10	-	expression tag
Leu 11	-	expression tag
Val 12	-	expression tag
Ile 13	-	expression tag
His 14	-	expression tag
Met 15	-	expression tag

C, G: 0 - 129 (UniProt: P04908)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

D, H: 0 - 125 (UniProt: P06899)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	202615.2
Non-Polymers*	Number of molecules	1
	Total formula weight	54.9
All*	Total formula weight	202670.1

*Water molecules are not included.