3W0M

Crystal structure of a thermostable mutant of aminoglycoside phosphotransferase APH(4)-Ia, apo form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Hygromycin-B 4-O-kinase	polymer	349	39076.8	1	UniProt (P00557) Pfam (PF01636) In PDB	Escherichia coli	APH(4), Hygromycin B phosphotransferase, Hygromycin-B kinase
2	A	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
3		water	water		18.0	245	Chemie (HOH)

Sequence modifications

A: 1 - 341 (UniProt: P00557)

PDB	External Database	Details
Gly 20	Asp 20	engineered mutation
Val 118	Ala 118	engineered mutation
Pro 225	Ser 225	engineered mutation
Leu 226	Gln 226	engineered mutation
Ala 246	Thr 246	engineered mutation
Leu 342	-	expression tag
Glu 343	-	expression tag
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	39076.8
Non-Polymers*	Number of molecules	1
	Total formula weight	118.2
All*	Total formula weight	39195.0

*Water molecules are not included.