3VW4

Crystal structure of the DNA-binding domain of ColE2-P9 Rep in complex with the replication origin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Rep	polymer	128	14727.0	2	UniProt (Q06B24) In PDB	Escherichia coli	replication initiator ColE2-Rep
2	C, E	DNA (5'-D(P*AP*AP*TP*GP*AP*GP*AP*CP*CP*AP*GP*AP*TP*AP*AP*GP*CP*CP*TP*TP*AP*TP*C)-3')	polymer	23	7057.6	2
3	D, F	DNA (5'-D(P*GP*AP*TP*AP*AP*GP*GP*CP*TP*TP*AP*TP*CP*TP*GP*GP*TP*CP*TP*CP*AP*TP*T)-3')	polymer	23	7061.6	2
4	A, B	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
5		water	water		18.0	27	Chemie (HOH)

Sequence modifications

A, B: 175 - 294 (UniProt: Q06B24)

PDB	External Database	Details
Met 167	-	expression tag
Gly 168	-	expression tag
His 169	-	expression tag
His 170	-	expression tag
His 171	-	expression tag
His 172	-	expression tag
His 173	-	expression tag
His 174	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	57692.3
Non-Polymers*	Number of molecules	5
	Total formula weight	480.3
All*	Total formula weight	58172.6

*Water molecules are not included.