3VUK

Crystal structure of a cysteine-deficient mutant M5 in MAP kinase JNK1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Mitogen-activated protein kinase 8	polymer	370	42645.1	1	UniProt (A1L4K2) Pfam (PF00069) UniProt (by SIFTS) (P45983)	Homo sapiens (human)	Mitogen-activated protein kinase 8 isoform JNK1 beta2, Mitogen-activated protein kinase 8, isoform CRA_d, cDNA FLJ77387, highly similar to Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant 4, mRNA
2	B (F)	Peptide from C-Jun-amino-terminal kinase-interacting protein 1	polymer	11	1345.6	1	UniProt (Q9UQF2)	Homo sapiens	JIP-1, JNK-interacting protein 1, Islet-brain 1, IB-1, JNK MAP kinase scaffold protein 1, Mitogen-activated protein kinase 8-interacting protein 1
3	C, D, E (A)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
4	F, G (A, F)	water	water		18.0	25	Chemie (HOH)

Sequence modifications

A: 1 - 364 (UniProt: A1L4K2)

PDB	External Database	Details
Val 79	Cys 79	engineered mutation
Ser 116	Cys 116	engineered mutation
Val 137	Cys 137	engineered mutation
Ala 163	Cys 163	engineered mutation
Ser 245	Cys 245	engineered mutation
His 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	43990.7
Non-Polymers*	Number of molecules	3
	Total formula weight	288.2
All*	Total formula weight	44278.9

*Water molecules are not included.