3VUH
Crystal structure of a cysteine-deficient mutant M3 in MAP kinase JNK1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Mitogen-activated protein kinase 8 | polymer | 370 | 42653.1 | 1 | UniProt (A1L4K2) Pfam (PF00069) UniProt (by SIFTS) (P45983) | Homo sapiens (human) | Mitogen-activated protein kinase 8 isoform JNK1 beta2, Mitogen-activated protein kinase 8, isoform CRA_d, cDNA FLJ77387, highly similar to Homo sapiens mitogen-activated protein kinase 8 (MAPK8), transcript variant 4, mRNA |
| 2 | B (F) | Peptide from C-Jun-amino-terminal kinase-interacting protein 1 | polymer | 11 | 1345.6 | 1 | UniProt (Q9UQF2) | Homo sapiens | JIP-1, JNK-interacting protein 1, Islet-brain 1, IB-1, JNK MAP kinase scaffold protein 1, Mitogen-activated protein kinase 8-interacting protein 1 |
| 3 | C, D, E, F, G (A) | SULFATE ION | non-polymer | 96.1 | 5 | Chemie (SO4) | |||
| 4 | H (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 5 | I (A) | water | water | 18.0 | 45 | Chemie (HOH) |
Sequence modifications
A: 1 - 364 (UniProt: A1L4K2)
| PDB | External Database | Details |
|---|---|---|
| Ser 116 | Cys 116 | engineered mutation |
| Ala 163 | Cys 163 | engineered mutation |
| Ser 245 | Cys 245 | engineered mutation |
| His 365 | - | expression tag |
| His 366 | - | expression tag |
| His 367 | - | expression tag |
| His 368 | - | expression tag |
| His 369 | - | expression tag |
| His 370 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 43998.7 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 572.4 | |
| All* | Total formula weight | 44571.1 |
