3VMT

Crystal structure of Staphylococcus aureus membrane-bound transglycosylase in complex with a Lipid II analog

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Monofunctional glycosyltransferase	polymer	263	30314.4	2	UniProt (Q99T05) Pfam (PF00912)	Staphylococcus aureus	MGT, Peptidoglycan TGase
2	C (A)	[(2R,3R,4R,5S,6R)-4-[(2R)-1-[[(2S)-1-[2-[2-[2-[5-[(3aS,4S,6aR)-2-oxidanylidene-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]ethylamino]-1-oxidanylidene-propan-2-yl]amino]-1-oxidanylidene-propan-2-yl]oxy-3-acetamido-5-[(2S,3R,4R,5R,6R)-3-acetamido-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl]oxy-6-(hydroxymethyl)oxan-2-yl] [oxidanyl(3,7,11,15,19,23,27,31,35,39,43-undecamethyltetratetraconta-2,6,10,14,18,22,26,30,34,38,42-undecaenoxy)phosphoryl] hydrogen phosphate	non-polymer		1833.3	1	Chemie (LHI)
3	D, E (A)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	F, G (A, B)	water	water		18.0	52	Chemie (HOH)

Sequence modifications

A, B: 28 - 269 (UniProt: Q99T05)

PDB	External Database	Details
Met 7	-	expression tag
Gly 8	-	expression tag
Ser 9	-	expression tag
Ser 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
Ser 17	-	expression tag
Ser 18	-	expression tag
Gly 19	-	expression tag
Leu 20	-	expression tag
Val 21	-	expression tag
Pro 22	-	expression tag
Arg 23	-	expression tag
Gly 24	-	expression tag
Ser 25	-	expression tag
His 26	-	expression tag
Met 27	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	60628.7
Non-Polymers*	Number of molecules	3
	Total formula weight	1881.9
All*	Total formula weight	62510.6

*Water molecules are not included.