3VDB
E. coli (lacZ) beta-galactosidase (N460T) in complex with galactonolactone
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Beta-galactosidase | polymer | 1052 | 119730.8 | 4 | UniProt (P00722) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) Pfam (PF16353) Pfam (PF02929) | Escherichia coli | Beta-gal, Lactase |
| 2 | CA, E, IB, JC (B, A, C, D) | D-galactonolactone | non-polymer | 178.1 | 4 | Chemie (149) | |||
| 3 | DA, EA, F, FA, G... (B, A, C, D) | MAGNESIUM ION | non-polymer | 24.3 | 9 | Chemie (MG) | |||
| 4 | GA, H, HA, I, IA... (B, A, C, D) | SODIUM ION | non-polymer | 23.0 | 14 | Chemie (NA) | |||
| 5 | AA, AB, AC, AD, BA... (A, B, C, D) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 77 | Chemie (DMS) | |||
| 6 | ED, FD, GD, HD (A, B, C, D) | water | water | 18.0 | 3458 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 9 - 1023 (UniProt: P00722)
| PDB | External Database | Details |
|---|---|---|
| Met -28 | - | expression tag |
| Gly -27 | - | expression tag |
| Gly -26 | - | expression tag |
| Ser -25 | - | expression tag |
| His -24 | - | expression tag |
| His -23 | - | expression tag |
| His -22 | - | expression tag |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| Gly -18 | - | expression tag |
| Met -17 | - | expression tag |
| Ala -16 | - | expression tag |
| Ser -15 | - | expression tag |
| Met -14 | - | expression tag |
| Thr -13 | - | expression tag |
| Gly -12 | - | expression tag |
| Gly -11 | - | expression tag |
| Gln -10 | - | expression tag |
| Gln -9 | - | expression tag |
| Met -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Arg -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Leu -4 | - | expression tag |
| Tyr -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Asp 0 | - | expression tag |
| Asp 1 | - | expression tag |
| Lys 2 | - | expression tag |
| Asp 3 | - | expression tag |
| Pro 4 | - | expression tag |
| Met 5 | - | expression tag |
| Ile 6 | - | expression tag |
| Asp 7 | - | expression tag |
| Pro 8 | - | expression tag |
| Thr 460 | Asn 461 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 478923.0 | |
| Non-Polymers* | Number of molecules | 104 |
| Total formula weight | 7269.4 | |
| All* | Total formula weight | 486192.4 |
