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3VDA

E. coli (lacZ) beta-galactosidase (N460T)

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A, B, C, D
(A, B, C, D)
Beta-galactosidasepolymer1052119730.84UniProt (P00722)
Pfam (PF02837)
Pfam (PF00703)
Pfam (PF02836)
Pfam (PF16353)
Pfam (PF02929)
Escherichia coliBeta-gal, Lactase
2CA, DA, E, F, NA...
(C, A, D, B)
MAGNESIUM IONnon-polymer24.38Chemie (MG)
3EA, FA, G, H, I...
(C, A, D, B)
SODIUM IONnon-polymer23.010Chemie (NA)
4AA, AB, BA, BB, GA...
(B, D, C, A)
DIMETHYL SULFOXIDEnon-polymer78.132Chemie (DMS)
5CB, DB, EB, FB
(A, B, C, D)
waterwater18.01611Chemie (HOH)
Sequence modifications
A, B, C, D: 9 - 1023 (UniProt: P00722)
PDBExternal DatabaseDetails
Met -28-expression tag
Gly -27-expression tag
Gly -26-expression tag
Ser -25-expression tag
His -24-expression tag
His -23-expression tag
His -22-expression tag
His -21-expression tag
His -20-expression tag
His -19-expression tag
Gly -18-expression tag
Met -17-expression tag
Ala -16-expression tag
Ser -15-expression tag
Met -14-expression tag
Thr -13-expression tag
Gly -12-expression tag
Gly -11-expression tag
Gln -10-expression tag
Gln -9-expression tag
Met -8-expression tag
Gly -7-expression tag
Arg -6-expression tag
Asp -5-expression tag
Leu -4-expression tag
Tyr -3-expression tag
Asp -2-expression tag
Asp -1-expression tag
Asp 0-expression tag
Asp 1-expression tag
Lys 2-expression tag
Asp 3-expression tag
Pro 4-expression tag
Met 5-expression tag
Ile 6-expression tag
Asp 7-expression tag
Pro 8-expression tag
Thr 460Asn 461engineered mutation
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains4
Total formula weight478923.0
Non-Polymers*Number of molecules50
Total formula weight2924.6
All*Total formula weight481847.6
*Water molecules are not included.

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PDB entries from 2024-11-20

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