3VDA
E. coli (lacZ) beta-galactosidase (N460T)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D (A, B, C, D) | Beta-galactosidase | polymer | 1052 | 119730.8 | 4 | UniProt (P00722) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) Pfam (PF16353) Pfam (PF02929) | Escherichia coli | Beta-gal, Lactase |
2 | CA, DA, E, F, NA... (C, A, D, B) | MAGNESIUM ION | non-polymer | 24.3 | 8 | Chemie (MG) | |||
3 | EA, FA, G, H, I... (C, A, D, B) | SODIUM ION | non-polymer | 23.0 | 10 | Chemie (NA) | |||
4 | AA, AB, BA, BB, GA... (B, D, C, A) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 32 | Chemie (DMS) | |||
5 | CB, DB, EB, FB (A, B, C, D) | water | water | 18.0 | 1611 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 9 - 1023 (UniProt: P00722)
PDB | External Database | Details |
---|---|---|
Met -28 | - | expression tag |
Gly -27 | - | expression tag |
Gly -26 | - | expression tag |
Ser -25 | - | expression tag |
His -24 | - | expression tag |
His -23 | - | expression tag |
His -22 | - | expression tag |
His -21 | - | expression tag |
His -20 | - | expression tag |
His -19 | - | expression tag |
Gly -18 | - | expression tag |
Met -17 | - | expression tag |
Ala -16 | - | expression tag |
Ser -15 | - | expression tag |
Met -14 | - | expression tag |
Thr -13 | - | expression tag |
Gly -12 | - | expression tag |
Gly -11 | - | expression tag |
Gln -10 | - | expression tag |
Gln -9 | - | expression tag |
Met -8 | - | expression tag |
Gly -7 | - | expression tag |
Arg -6 | - | expression tag |
Asp -5 | - | expression tag |
Leu -4 | - | expression tag |
Tyr -3 | - | expression tag |
Asp -2 | - | expression tag |
Asp -1 | - | expression tag |
Asp 0 | - | expression tag |
Asp 1 | - | expression tag |
Lys 2 | - | expression tag |
Asp 3 | - | expression tag |
Pro 4 | - | expression tag |
Met 5 | - | expression tag |
Ile 6 | - | expression tag |
Asp 7 | - | expression tag |
Pro 8 | - | expression tag |
Thr 460 | Asn 461 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 478923.0 | |
Non-Polymers* | Number of molecules | 50 |
Total formula weight | 2924.6 | |
All* | Total formula weight | 481847.6 |