3VBM

Crystal Structure of the S84T mutant of AntD, an N-acyltransferase from Bacillus cereus in complex with dTDP and Coenzyme A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, C, E)	Galactoside O-acetyltransferase	polymer	205	22766.3	3	UniProt (D7WGJ0)	Bacillus cereus	AntD
2	D, E, H (A, C)	COENZYME A	non-polymer		767.5	3	Chemie (COA)
3	F, J, L (A, C, E)	THYMIDINE-5'-DIPHOSPHATE	non-polymer		402.2	3	Chemie (TYD)
4	G, K, M (A, C, E)	BICARBONATE ION	non-polymer		61.0	3	Chemie (BCT)
5	I (C)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	N, O, P (A, C, E)	water	water		18.0	290	Chemie (HOH)

Sequence modifications

A, C, E: 1 - 188 (UniProt: D7WGJ0)

PDB	External Database	Details
Met -16	-	expression tag
Gly -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
His 0	-	expression tag
Thr 84	Ser 84	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	68299.0
Non-Polymers*	Number of molecules	10
	Total formula weight	3727.7
All*	Total formula weight	72026.6

*Water molecules are not included.