3V7K

Co-crystal structure of K72E variant of rat polymerase beta: Enzyme-DNA binary complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase beta	polymer	340	39058.4	1	UniProt (P06766) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791)	Rattus norvegicus (rat)
2	B (P)	DNA (5'-D(P*AP*TP*GP*TP*GP*AP*GP*T)-3')	polymer	8	2481.7	1
3	C (T)	DNA (5'-D(P*CP*AP*AP*AP*CP*TP*CP*AP*CP*AP*A)-3')	polymer	11	3295.2	1
4	D, E, F, G (A)	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
5	H, I, J (A, P, T)	water	water		18.0	127	Chemie (HOH)

Sequence modifications

A: 4 - 335 (UniProt: P06766)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
Glu 72	Lys 72	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	44835.2
Non-Polymers*	Number of molecules	4
	Total formula weight	92.0
All*	Total formula weight	44927.2

*Water molecules are not included.