3V61

Structure of S. cerevisiae PCNA conjugated to SUMO on lysine 164

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ubiquitin-like protein SMT3	polymer	84	9720.0	1	UniProt (Q12306) Pfam (PF00240) In PDB	Saccharomyces cerevisiae (Baker's yeast)
2	B	Proliferating cell nuclear antigen	polymer	258	28871.9	1	UniProt (P15873) Pfam (PF00705) Pfam (PF02747) In PDB	Saccharomyces cerevisiae (Baker's yeast)	PCNA
3	A, B	BARIUM ION	non-polymer		137.3	13	Chemie (BA)
4	B	N-ETHYLMALEIMIDE	non-polymer		125.1	2	Chemie (NEQ)
5		water	water		18.0	90	Chemie (HOH)

Sequence modifications

A: 20 - 98 (UniProt: Q12306)

PDB	External Database	Details
Gly 15	-	expression tag
Ser 16	-	expression tag
His 17	-	expression tag
Met 18	-	expression tag
Arg 19	-	expression tag

B: 1 - 258 (UniProt: P15873)

PDB	External Database	Details
Gly 127	Lys 127	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	38591.9
Non-Polymers*	Number of molecules	15
	Total formula weight	2035.5
All*	Total formula weight	40627.4

*Water molecules are not included.