3V4Y
Crystal Structure of the first Nuclear PP1 holoenzyme
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E, G (A, C, E, G) | Serine/threonine-protein phosphatase PP1-alpha catalytic subunit | polymer | 306 | 34941.1 | 4 | UniProt (P62136) Pfam (PF16891) Pfam (PF00149) | Homo sapiens (human) | PP-1A |
| 2 | B, D, F, H (B, D, F, H) | Nuclear inhibitor of protein phosphatase 1 | polymer | 62 | 7213.8 | 4 | UniProt (Q12972) | Homo sapiens (human) | NIPP-1, Protein phosphatase 1 regulatory inhibitor subunit 8, Activator of RNA decay, ARD-1 |
| 3 | I, J, O, P, T... (A, C, E, G) | MANGANESE (II) ION | non-polymer | 54.9 | 8 | Chemie (MN) | |||
| 4 | AA, K, L, M, Q... (G, A, C, E) | GLYCEROL | non-polymer | 92.1 | 10 | Chemie (GOL) | |||
| 5 | N (A) | POLYETHYLENE GLYCOL (N=34) | non-polymer | 1529.8 | 1 | Chemie (15P) | |||
| 6 | BA, CA, DA, EA, FA... (A, B, C, D, E...) | water | water | 18.0 | 661 | Chemie (HOH) |
Sequence modifications
A, C, E, G: 7 - 307 (UniProt: P62136)
B, D, F, H: 158 - 216 (UniProt: Q12972)
| PDB | External Database | Details |
|---|---|---|
| Gly -5 | - | expression tag |
| His -4 | - | expression tag |
| Met -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Met -1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 168619.6 | |
| Non-Polymers* | Number of molecules | 19 |
| Total formula weight | 2890.3 | |
| All* | Total formula weight | 171509.9 |
