3V47
Crystal structure of the N-terminal fragment of zebrafish TLR5 in complex with Salmonella flagellin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Toll-like receptor 5b and variable lymphocyte receptor B.61 chimeric protein | polymer | 455 | 51169.2 | 2 | UniProt (B3DIN1) UniProt (Q4G1L2) Pfam (PF13855) Pfam (PF11921) | Danio rerio (zebra fish inshore hagfish) | |
| 2 | C, D (C, D) | Flagellin | polymer | 425 | 44548.7 | 2 | UniProt (Q06971) Pfam (PF00669) Pfam (PF22370) Pfam (PF00700) | Salmonella enterica subsp. enterica serovar Dublin | Phase 1-C flagellin |
| 3 | E, F, G, H (E, F, G, H) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 4 | In PDB GlyTouCan (G42666HT) | |||
| 4 | I, J, K (A, B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 3 | Chemie (NAG) | |||
| 5 | L, M, N, O (A, B, C, D) | water | water | 18.0 | 291 | Chemie (HOH) |
Sequence modifications
A, B: 22 - 390 (UniProt: B3DIN1)
A, B: 391 - 465 (UniProt: Q4G1L2)
C, D: 47 - 465 (UniProt: Q06971)
| PDB | External Database | Details |
|---|---|---|
| Ala 18 | - | expression tag |
| Asp 19 | - | expression tag |
| Pro 20 | - | expression tag |
| Gly 21 | - | expression tag |
| Glu 24 | Val 24 | engineered mutation |
| Val 124 | Leu 124 | engineered mutation |
| Lys 159 | Gln 159 | engineered mutation |
| Lys 227 | Arg 227 | engineered mutation |
| Thr 229 | Ser 229 | engineered mutation |
| Asn 334 | Asp 334 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser 466 | - | expression tag |
| Ala 467 | - | expression tag |
| Ser 468 | - | expression tag |
| Leu 469 | - | expression tag |
| Val 470 | - | expression tag |
| Pro 471 | - | expression tag |
| Arg 472 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 41 | - | expression tag |
| Ser 42 | - | expression tag |
| Ala 43 | - | expression tag |
| Lys 44 | - | expression tag |
| Asp 45 | - | expression tag |
| Pro 46 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 191435.8 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 1697.6 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 663.6 | |
| All* | Total formula weight | 193797.0 |
