3V3X
Nitroxide Spin Labels in Protein GB1: N8/K28 Double Mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Immunoglobulin G-binding protein G | polymer | 56 | 6191.8 | 4 | UniProt (P19909) Pfam (PF01378) | Streptococcus sp. 'group G' | IgG-binding protein G |
| 2 | E, G, H, K, L... (A, B, C, D) | S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate | non-polymer | 264.4 | 7 | Chemie (MTN) | |||
| 3 | F (A) | NONAETHYLENE GLYCOL | non-polymer | 414.5 | 1 | Chemie (2PE) | |||
| 4 | I (B) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 5 | J, O (B, D) | ACETATE ION | non-polymer | 59.0 | 2 | Chemie (ACT) | |||
| 6 | P, Q, R, S (A, B, C, D) | water | water | 18.0 | 129 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 3 - 56 (UniProt: P19909)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | expression tag |
| Gln 2 | - | expression tag |
| Cys 8 | Asn 309 | engineered mutation |
| Cys 28 | Lys 329 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 24767.2 | |
| Non-Polymers* | Number of molecules | 11 |
| Total formula weight | 2475.4 | |
| All* | Total formula weight | 27242.6 |
